Abstract
This study developed a method for simultaneous determination of 13 elements of Semen Cuscutae (quercitrin, quercetin, hyperoside, caffeic acid, chlorogenic acid, luteolin, apigenin, kaempferol, isoquercitrin, cryptochlorogenic acid, isorhamnetin-3-O-glucoside, astragalin, and rutin) in rat plasma using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) in the negative MRM mode. The analytes were analyzed with CORTECS®C18 column (4.6 × 150 mm, 2.7 μm) with mobile phases consisting of 0.1% formic acid in water (A) and acetonitrile (B). The intra- and interday precision of the target compounds were expressed as relative standard deviation (RSD) in the range of 0.5%–10.4%, and the accuracy of the target compounds was expressed as relative error (RE) not exceeding ±14.5% for all analytes. In the meantime, the extraction recovery of the target compounds in plasma samples ranged from 87.4% to 106.2% and matrix effect from 81.0% to 115.5%. The established method was successfully accomplished for the pharmacokinetic study of the analytes in rat plasma samples following oral administration of Semen Cuscutae extract, and the pharmacokinetic parameters of seven compounds were obtained.
Highlights
Semen Cuscutae (Longxuzi, Tusizi), the dry mature seed, belongs to Cuscuta australis R.Br. or Cuscuta chinensis Lam. of Convolvulaceae family [1, 2]
In order to improve sensitivity and shorten analysis time, we tested different columns and various mobile phase systems. Comparing different columns such as CORTECS C18 column (4.6 mm × 150 mm, 2.7 μm), TM ® Xbridge C18 column (2.1 mm × 150 mm, 3.5 μm), and TM Xbridge C18 column (4.6 mm × 50 mm, 2.5 μm), and various mobile phase systems such as acetonitrile-water or 0.1% formic acid in water and methanol-water or 0.1% formic acid in water, we found that the sensitivity and signal response of
E standard solutions of the target compounds and IS were infused into the instrument separately, both positive and negative ion modes were compared to optimize mass conditions. e results showed that all analytes were better eluted under negative ionization mode
Summary
Semen Cuscutae (Longxuzi, Tusizi), the dry mature seed, belongs to Cuscuta australis R.Br. or Cuscuta chinensis Lam. of Convolvulaceae family [1, 2]. It was first recorded in the “Shen Nong’s Herbal” as an upper grade drug [1]. Qualitative and quantitative analysis of its components in traditional Chinese medicine has certain significance. The chemical studies on Semen Cuscutae have mainly focused on qualitative analysis of its major components using different analytical methods, such as HPLC, LC-MS/MS, and so on [12], but the pharmacokinetic studies of Semen Cuscutae were rarely reported. Zhang et al [13] established the kidney-deficiency model on rats to expound the pharmacokinetic differences of six renoprotective compounds
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