Abstract

Phenotyping is a critical process in plant breeding, especially when there is an increasing demand for streamlining a selection process in a breeding program. Since manual phenotyping has limited efficiency, high-throughput phenotyping methods are recently popularized owing to progress in sensor and image processing technologies. However, in a size-limited breeding field, which is common in Japan and other Asian countries, it is challenging to introduce large machinery in the field or fly unmanned aerial vehicles over the field. In this study, we developed a ground-based high-throughput field phenotyping rover that could be easily introduced to a field regardless of the scale and location of the field even without special facilities. We also made the field rover open-source hardware, making its system available to public for easy modification, so that anyone can build one for their own use at a low cost. The trial run of the field rover revealed that it allowed the collection of detailed remote-sensing images of plants and quantitative analyses based on the images. The results suggest that the field rover developed in this study could allow efficient phenotyping of plants especially in a small breeding field.

Highlights

  • High throughput field phenotyping is a key technology for accelerating plant science, both in basic science for largescale genetic and physiological research projects and in agricultural application such as streamlining selection processes in breeding programs (Araus and Cairns 2014)

  • We developed a field phenotyping rover for sensing a size-limited breeding field as open-source hard‐ ware

  • Our field rover was able to acquire a rich amount of image data of growing wheat

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Summary

Introduction

High throughput field phenotyping is a key technology for accelerating plant science, both in basic science for largescale genetic and physiological research projects and in agricultural application such as streamlining selection processes in breeding programs (Araus and Cairns 2014). Conventional manual pheno‐ typing methods are generally labor-intensive or costly due to their inherently limited efficiency. This limitation in the efficiency of phenotyping has become a major bottle‐. Neck of genome-phenotype association methods, such as Genome-Wide Association Studies and Genomic Selection, and resulted in a strong demand for more efficient pheno‐ typing methods (Furbank and Tester 2011). To increase the efficiency as well as accuracy in pheno‐ typing, researchers have developed various high-throughput phenotyping methods. Systems with belt conveyer were developed for indoor environment cultivations to automate and accelerate pheno‐ typing (Fujita et al 2018, Tisné et al 2013). For cultiva‐ tions in outdoor environments, where introducing such a complex system is challenging, large gantry cranes equipped with various sensors have been deployed

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