Abstract
This article describes a complete micropropagation protocol employing node segments from branches of adult trees of ‘Swingle’ citrumelo. Multiple shoots were induced in vitro through enhanced axillary branching. A multiplication rate of 3.79-fold every four weeks was achieved on Murashige and Skoog's media (1962) supplemented with cytokinins (6-benzyl amino purine and kinetin) with 4% sucrose and 0.4% agargel. The highest percent rooting was observed with a combination of indole-3-acetic acid and indole-3-butyric acid. The hardening survival rate of the rooted plantlets was 100% in a soil and agropeat combination. The micropropagated plantlets were tested for their genetic fidelity using seven inter simple sequence repeats primers.
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