Development of a high polarity-sensitive fluorescent probe for visualizing the lipid droplets and endoplasmic reticulum with dual colors in living cells

Abstract

Lipid droplets (LDs) are unique organelles that control the lipid metabolism in cells. It has been identified that the generations of LDs derive from endoplasmic reticulum (ER) and they have closely related with amount of cellular activities for maintaining homeostasis. To further explore the detail interactions between LDs and ER, we have developed a novel polarity-sensitive fluorescent probe LP with distinct D-π-A-π-D framework and applied it to imaging LDs and ER with dual colors at the same time. Probe LP showed well red-shifted emissions with the increase fraction of water in the 1,4- dioxane due to ICT process. In biological imaging, probe LP could visualize LDs and ER with green and red fluorescence separately. Besides, the dynamic behaviors of LDs and ER were achieved using LP during the oleic acids and starvation stimulations. Therefore, probe LP is a valuable molecular tool for investigating the relationships of LDs and ER in various cellular activities.