Abstract
We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.
Highlights
Optical manipulation and trapping of microscopic particles, single molecules and cells has allowed for a wide range of powerful biophysics studies [1, 2]
We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens
This enables optical trapping at a distance of 200 μm from the probe tip
Summary
Optical manipulation and trapping of microscopic particles, single molecules and cells has allowed for a wide range of powerful biophysics studies [1, 2]. Graded index (GRIN) fibers have been presented as an alternative approach for generating a tight focus near the fiber tip, allowing optical trapping at a distance between 3 and 10 μm from the fiber tip [10], and have recently been utilized to extend the optical manipulation length of the fiber probe to 40 μm and even up to 60 μm under certain environmental conditions [11]. These approaches require fabrication of GRIN fibers with specially tuned geometric parameters which limits accessibility. To the best of our knowledge, this is the first demonstration of the use of an optical fiber trapping probe for the manipulation of immune cells
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