Abstract
Isoprothiolane (IPT) is widely used to control rice blast, but can cause unacceptable harm to organisms making its detection in rice crucial. In this work, a hapten based on the structure of IPT was produced to prepare a monoclonal antibody (mAb) with high sensitivity and specificity. The half maximum inhibitory concentration of the anti-IPT mAb was 0.81 ng/mL as determined by ELISA, and the cross reactivity with analogues was negligible. A GNP-based lateral flow immunoassay (LFIA) method was subsequently developed to quantitatively detect IPT in brown and polished rice. The optimized LFIA, in brown rice samples, showed a visual limit of detection, cut-off value and calculated limit of detection of 2 mg/kg, 10 mg/kg and 81 ug/kg, respectively and 0.2 mg/kg, 4 mg/kg and 66 ug/kg in polished rice samples. Recovery rates in spiked brown and polished rice were 95.21 %–103.06 % and 91.05 %–104.13 %, with a coefficient of variation of 1.25 %–4.17 % and 2.93 %–8.42 %, respectively. No significant difference in recovery rates were seen between LFIA and LC-MS/MS indicating that the prepared GNP-based LFIA had good potential for the detection of IPT in rice.
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