Development of a dynamic feeding strategy for continuous-flow aerobic granulation and nitrogen removal in a modified airlift loop reactor for municipal wastewater treatment

Abstract

This study investigated the aerobic sludge granulation and nitrogen removal performance in a modified airlift loop reactor treating municipal wastewater under different operation conditions. Dynamic feeding and aeration control were applied to create feast/famine conditions to facilitate microbial aggregation. Experimental results demonstrated that aerobic granular sludge could be cultivated in continuous-flow reactors fed with an optimized dynamic feeding condition. Fresh granules sizing 0.4–0.6 mm were observed in the reactors after a 61-day operation, then turned to matured granules after another 33-day operation with a compact structure, a stable size of 2–4 mm, and a low SVI of ~35 mL/g. Extracellular polymeric substances (EPS) analysis results showed that both EPS contents and the ratio of protein to polysaccharides increased with the granulation process, leading to an increase of cell hydrophobicity. Granular sludge exhibited a good nitrogen removal ability with a comparable level of specific nitrification rate and denitrification rate with those measured in state-of-the-art sequential batch reactors. Microbial population analysis showed an increase in the relative abundance of functional microbes, including Zoogloea, Nitrospira, Dechloromonas, and Thauera in the cultivated granules, suggesting a potentially crucial role of these microbes in sludge granulation and nitrogen removal. The dynamic feeding strategy and the reactor configuration are considered as critical factors for aerobic granulation under continuous-flow conditions for creating feast/famine conditions and allow sludge backflow without structure damage.