Abstract
: A competitive direct enzyme-linked immunosorbent assay (dc-ELISA) was developed for the detection and quantification of scopolamine (SCO) in wheat flours and cereal samples (multigrain, oat and barley). The limit of quantification (IC20) of the established method was 6.00 ± 1.20 ng/g, with the limit of detection (IC10) being 2.4 ± 0.6 ng/g in wheat flour with a coefficient of variation (CV) less than 20%. The assay was highly specific to SCO and nor-scopolamine, with no cross-reactivity to other similar structures. In spiked wheat flours the recoveries ranged from 84% to 104% with CVs of less than 20% and the recovery from a Food Analysis Performance Assessment Scheme (FAPAS) buckwheat control sample was 118%. A comparison of spiked wheat flour and a FAPAS control sample showed similar results to those determined by classical LC-MS/MS methods. A small retail survey of wheat flours and cereal samples was conducted using this ELISA method and a LC-MS/MS method, which showed scopolamine was not detected in any of these survey samples by either method. This method is suitable for rapid quantitation of SCO in wheat flours and cereal samples.
Published Version
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