Development of a denitrification system using primary sludge as solid carbon source – Potential to couple with anammox process

Abstract

Heterotrophic denitrification is a robust and reliable process for nitrogen removal from wastewater. However, wastewater often faces the issue of lacking carbon source. In this study, the feasibility of using primary sludge, a by-product of wastewater treatment plants, to support denitrification of high-strength nitrite wastewater was investigated. Results suggest the desired performance can be achieved with the influent nitrite concentration of 400 to 1200 mg N/L, and the optimal primary sludge dosage for the complete nitrite removal was 3.6 g VSS/g N. Ammonium removal was also detected along with nitrite removal. Microbial analysis reveals various types of denitrifying bacteria and a large number of macromolecular organics degrading bacteria existed in the microbial community. Notably, anammox bacteria, Candidatus Brocadia, was also identified with an abundance of 0.1%. The slow kinetics of carbon source release from primary sludge was likely the reason for the existence of anammox process. This study developed a promising nitrogen removal process using an alternative carbon source for denitrification, and it shows great potential to couple denitrification with anammox to reduce ammonium residue.