Development of a Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method.

Kanaporn Poltep,Akio Okubo,Nathamon Kosoltanapiwat,Pornsawan Leaungwutiwong,Narinee Srimark,Takeshi Kurosu,Chiaki Kitamura,Tatsuo Shioda,Sarin Suwanpakdee,Yoshiki Takashima,Juthamas Phadungsombat,Tadahiro Sasaki,Atsushi Yamanaka,Hisham A Imad,Borimas Hanboonkunupakarn,Emi E Nakayama

doi:10.3390/s21237809

Abstract

Four serotypes of dengue virus (DENV), type 1 to 4 (DENV-1 to DENV-4), exhibit approximately 25–40% of the difference in the encoded amino acid residues of viral proteins. Reverse transcription of RNA extracted from specimens followed by PCR amplification is the current standard method of DENV serotype determination. However, since this method is time-consuming, rapid detection systems are desirable. We established several mouse monoclonal antibodies directed against DENV non-structural protein 1 and integrated them into rapid DENV detection systems. We successfully developed serotype-specific immunochromatography systems for all four DENV serotypes. Each system can detect 104 copies/mL in 15 min using laboratory and clinical isolates of DENV. No cross-reaction between DENV serotypes was observed in these DENV isolates. We also confirmed that there was no cross-reaction with chikungunya, Japanese encephalitis, Sindbis, and Zika viruses. Evaluation of these systems using serum from DENV-infected individuals indicated a serotype specificity of almost 100%. These assay systems could accelerate both DENV infection diagnosis and epidemiologic studies in DENV-endemic areas.

Highlights

Dengue is a serious disease of public importance, with increasing worldwide spread [1].The disease is caused by infection with the dengue virus (DENV)
Some reports suggest that disease severity varies among DENV serotypes [3]
We describe viral non-structural protein 1 (NS1) antigen–based rapid tests that use monoclonal antibody (MAb) pairs to detect and distinguish the four DENV

Summary

Introduction

Dengue is a serious disease of public importance, with increasing worldwide spread [1]. The disease is caused by infection with the dengue virus (DENV). Since the first isolation of DENV in 1943, four antigenically distinct serotypes (DENV-1–4) have been identified. Global phenomena such as urbanization and increased international travel are key factors facilitating the spread of dengue. Documenting the type-specific spread of DENV could have important implications in understanding patterns in dengue hyperendemicity and 4.0/). Disease severity, as well as vaccine design and deployment strategies [2]. Some reports suggest that disease severity varies among DENV serotypes [3]

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