Abstract
Hypopharynx reconstruction after hypopharyngectomy is still a great challenge. Perfusion decellularization is for extracellular matrix (ECM) scaffolding and had been used in organ reconstruction. Our study aimed to prepare an acellular, natural, three-dimensional biological hypopharynx with vascular pedicle scaffold as the substitute materials to reconstruct hypopharynx. Scanning electron microscope and histology staining showed that the decellularized hypopharynx with vascular pedicle scaffold retained intact native anatomical ECM structure. Myoblasts were observed on the recellularized scaffolds with bone marrow mesenchymal stem cells induced by 5-azacytidine implanted in the rabbit greater omentum by immunohistochemical analysis. The decellularized hypopharynx with vascular pedicle scaffold prepared by detergent perfusion in our study has a potential to be an alternative material to pharynx reconstruction.
Highlights
Version of Record: A version of this preprint was published at Arti cial Organs on February 22nd, 2022
The results indicated that the composite scaffold retained intact native anatomical extracellular matrix (ECM) structure and component by hematoxylin and eosin (HE) staining and Scanning electron microscope (SEM) and low immunogenicity by immunofluorescence
The images of the decellularized tissue indicated that it is efficient to remove the majority of the cellular element by perfusing detergent
Summary
Version of Record: A version of this preprint was published at Arti cial Organs on February 22nd, 2022. Hypopharyngeal squamous cell carcinoma (HSCC) accounts for approximately 3% to 5% of all head and neck squamous cell carcinomas (HNSCC), and is less prevalent than most other major HNSCC, such as the laryngeal cancer and oral squamous cell carcinomas [1, 2] These patients with HSCC have worse prognosis than other types of HNSCC since the tumor in these patients has developed into stage III or IV when diagnosed [1, 3, 4]. Common artificial biomaterials included collagen, fibrin, silk, alginate and so on These materials are lack of the structural, chemical, biological, and mechanical complexity of native ECM [20]. Ott et al prepared a acellular, natural, three-dimensional (3D) biological cardiac scaffold retaining the natural structure by perfusion and decellularization, and successfully simulated cardiac tissue with a certain contraction function [26]. There is no report using decellularized hypopharynx with vascular pedicle to solve the defect after hypopharyngectomy
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