Development of a Cross-Linked Polysaccharide of Ligusticum wallichii – Squid Skin Collagen Scaffold Fabrication and Property Studies for Tissue-Engineering Applications

Abstract

Porous composite scaffold with Ligusticum wallichii polysaccharide and squid skin collagen was synthesized and characterized. The composite scaffold showed good phosphate buffer solution (PBS) retention ability, slow biodegradation rate, high mechanical strength, and superb cell proliferation promoting ability. The PBS content value increased from 433.40% to 571.05%; the weight lost in seven days decreased from 95.41% to 34.35%; the tensile strength of sample increased from 0.19 to 0.81 MPa; the seven-day relative growth rate of cell in composite (polysaccharide:collagen = 5:5) is twice of that in pure collagen. While with the polysaccharide portion increased, the elongation at break decreased from 93.09% to 9.60%.