Abstract
A simple, inexpensive, and quick colorimetric immunoassay for the determination of diabetic biomarker (glycated albumin) using antibody labeled gold nanoparticles is reported. The sensitivity of the assay on the basis of absorbance measurements is excellent with a detection limit of 1 μg/ml with 40 nm sized gold nanoparticles. The assay is free of washing cycle and result can be read in minutes. Also, the method requires very low amount of the sample as compared to the conventional immunossays. Therefore, this method can be used for the simple, low cost, and rapid detection of diabetes marker, glycated albumin.
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