Abstract
A chemiluminescent acridinium salt has been used to label a monoclonal antibody to hepatitis B surface antigen HBsAg). Since the antibody recognises a repeating epitope on HBsAg the same antibody in its unlabelled form has been used to coat the wells of a 96-well microtitre plate. These reagents have been used as the basis of a two-site immunochemiluminometric assay (ICMA) for the detection of HBsAg in serum. The assay consists of a single 90 minute incubation at 37°C followed by a wash step and tehn quantitation of chemiluminescence intensity in a microtitre late luminometer. The counting time is 1 second per well and the assay has a sensitivity of detection of 0.07 ng ml −1.
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