Abstract
Background The development of safe and effective topical microbicides to limit the continuing AIDS pandemic is a high priority. The current NIAID microbicide testing algorithm includes both CCR5 (R5)-tropic and CXCR4 (X4)-tropic attachment inhibition assays, and an X4-tropic fusion inhibition assay. To complete this testing algorithm, an R5-tropic fusion inhibition assay was developed. The rationale for this development was that mucosal HIV-1 infection is primarily mediated through the R5 receptor and an R5-tropic entry inhibitor should be included in combination microbicide prophylaxis.
Highlights
The development of safe and effective topical microbicides to limit the continuing AIDS pandemic is a high priority
The current NIAID microbicide testing algorithm includes both CCR5 (R5)-tropic and CXCR4 (X4)-tropic attachment inhibition assays, and an X4-tropic fusion inhibition assay. To complete this testing algorithm, an R5-tropic fusion inhibition assay was developed. The rationale for this development was that mucosal HIV-1 infection is primarily mediated through the R5 receptor and an R5-tropic entry inhibitor should be included in combination microbicide prophylaxis
In order to determine suitability for high throughput screening, the robustness of the assay was assessed by determining the Z'-value in a 96-well plate format, yielding a Z'-value of 0.7
Summary
Address: 1Southern Research Institute, Frederick, Maryland, 21701, USA, 2Southern Research Institute, Birmingham, Alabama, 35205, USA and 3Institut Cochin, INSERM U567, Paris, France. A single PDF containing all abstracts in this Supplement is available here http://www.biomedcentral.com/content/pdf/1742-4690-3-S1-info.pdf
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