Abstract
We developed a single reaction, fluorescently labeled in-house STR panel containing 19 autosomal loci plus Amelogenin for forensic use. We chose 18 loci from two commercial kits (AmpFlSTR® Identifiler®, Thermo Fisher Scientific, and The PowerPlex® 16 System, Promega) and combined with two mini-STRs (D6S1043 and D12S391) that do not exist in either of the kits to constitute a 20plex panel. The 20plex system is evaluated by specificity, sensitivity, precision and accuracy, analytical threshold, stochastic threshold, heterozygous balance, case-type samples, and PCR-based studies. The results demonstrate that 20plex STR multiplex panel is a robust, reliable and valuable assay for forensic applications.
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