Abstract
Populus L. is a tree genus that includes species with a high ability for interspecies hybridization. This process takes place in nature, and is used in poplar breeding. As а result, species identification in poplar populations and plantations is very difficult. In this study, a molecular marker system was developed for the identification of the most significant poplar species (P. nigra L. and P. deltoids Bartr. ex Marshall). The basis of the system is a polymorphism in non-transcribed spacers (NTSs) of 5S rDNA. The species-specific molecular markers were tested on a number of species and hybrids of poplars. It was shown that the marker system is a powerful tool for species identification, hybrid analysis, parent identification, and poplar breeding.
Highlights
Poplars (Populus L. species) are widely-used forest trees that have strategic interest for many countries in the Northern Hemisphere
We present another approach to identifying poplars
Nigra and P. deltoides 5S rDNA sequences were found in GenBank and used for non-transcribed spacers (NTSs) isolation
Summary
Poplars (Populus L. species) are widely-used forest trees that have strategic interest for many countries in the Northern Hemisphere. Their significant role in national economics was the reason for their study by chemists, agronomists, geneticists, breeders, and others [1,2,3]. Hybridization successfully occurs among species in the same section and between some sections [5,6,7]. This feature of poplars has long been used in breeding programs. The distinction of species and hybrids, and the determination of parents in hybrid poplars is almost impossible by morphological analysis alone
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