Development of 21 novel microsatellite markers for conservation genetic studies of Brachymystax lenok tsinlingensis, an endangered species in Korea, using next-generation sequencing

Abstract

Using a next-generation sequencing approach, we have developed 21 novel microsatellite markers in Brachymystax lenok tsinlingensis, an endangered freshwater fish species in Korea. All new microsatellite markers were successfully genotyped using 30 samples from 2 tributaries (BA and DMZ) of the Han River. These new microsatellite markers produced 102 alleles, varying from 2 to 9 alleles per locus in each tributary. No significant deviation from Hardy–Weinberg equilibrium or linkage disequilibrium in marker-pair/population was observed between markers, except for BleDi55. These markers developed can be valuable tools to conservation genetics of this species across its distributional range.