Abstract
Twenty microsatellite markers were isolated and characterized for Solenocera crassicornis from a (GT)13-enriched genomic library. Their polymorphisms were investigated using 44 wild individuals from the South Yellow Sea. Our investigation revealed that all the markers were polymorphic. The number of alleles per locus varied from 6 to 19 with an average of 12.35. The observed and expected heterozygosities ranged from 0.400 to 0.977 and from 0.609 to 0.940, with averages of 0.788 and 0.859, respectively. Four loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni’s correction. Cross-species amplification was also conducted in Solenocera melantho collected from the East China Sea. The result showed that 14 loci could be amplified from Solenocera melantho DNAs. These polymorphic markers would be useful for assessment of genetic variation and population structure of S. crassicornis and S. melantho.
Highlights
Solenocera crassicornis is an important prawn species in fishery resource and is widely distributed in the adjacent waters of Singapore, Indonesia, India, and the South Yellow Sea and the East Sea of China [1]
It is necessary and important to carry out research on S. crassicornis to promote the effective conservation and sustainable development of this prawn species
We developed the microsatellite loci in S. crassicornis and characterized the microsatellite markers by genotyping 44 individuals sampled from a wild population
Summary
Solenocera crassicornis is an important prawn species in fishery resource and is widely distributed in the adjacent waters of Singapore, Indonesia, India, and the South Yellow Sea and the East Sea of China [1]. S. crassicornis, along with Parapenaeopsis hardwickii, Trachypenaeus curvirostris, Metapenaeopsis dalei, and Palaemon gravieri, constitute the five major fishing prawn species of the Yellow Sea and East China Sea of China. Their catches occupied 80%~90% of the total production in the waters’ dragged prawn resources, and created high economic value [2,3,4]. This study would provide a technical support to investigate and evaluate the status of the genetic resources of S. crassicornis
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