Abstract
Quercus gilva is an evergreen oak species which is native to Japan, continental China and the islands of Taiwan. However, because of historical logging practices and habitat destruction in ancient times, Q. gilva is threatened with extinction in several regions of Japan. In this study, 13 polymorphic markers spanning the whole chloroplast DNA of Q. gilva were developed, using next-generation sequencing technology. These markers could distinguish between two individuals established in close proximity to one another as well as those from geographically-distinct regions. This indicates that the markers will be suitable for detecting the genetic structure and phylogeography of Q. gilva on both a large and a fine scale, potentially providing us with useful information for the conservation of this species.
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