Development of 11β -Hydroxysteroid Dehydrogenase Expression in the Rat Cochlea

Abstract

11β-hydroxysteroid dehydrogenase (11β-HSD) catalyzes the transformation of active glucocorticoid (GC) steroids to inactive 11-oxosteroids, as well as the reverse reaction. 11β-HSD was previously demonstrated specifically in the spiral ligament of the lateral cochlear wall where it was co-localized with adrenal steroid receptors. These findings imply that 11 β-HSD regulates binding of corticoids to their inner ear receptors. The GC receptor expression initially occurs around the critical maturation period of the cochlear duct. 11 β-HSD, which is an integral part of the cochlear steroid receptor system, could indirectly affect glucocorticoid-mediated induction processes. In this study the expression of 11β-HSD was studied in the postnatal rat cochlea from the 3rd to 30th postnatal day. Bouin's fixed, paraffin-embedded cochlear sections were processed for immunocytochemical detection of 11β-HSD using polyclonal antibodies against 11 β-HSD. 11β-HSD expression appeared at the 12th postnatal day at low levels in spiral ligament tissues. From the 15th postnatal day, 11β-HSD expression was stronger and similar to that of the adult cochlea. No additional inner ear tissue region expressed 11 β-HSD enzyme during the observed period. 11β-HSD expression coincides with the onset of functional maturity of the rat cochlear duct. The expression of 11β-HSD is preceded by the expression of GC receptors which appeared at the 7th postnatal day in the rat cochlea. These results further suggest an integrative role of the cochlear steroid receptor system in the homeostasis and functional maturation of the cochlea.