Development of 1,5-Diaryl-Pyrazole-3-Formate Analogs as Antifungal Pesticides and Their Application in Controlling Peanut Stem Rot Disease.

Yihui Ma,Jun Yang,Dangwei Yang,Guangyu Qin,Junhuai Zu

doi:10.3389/fmicb.2021.728173

Yihui Ma, Jun Yang + Show 3 more

Open Access

https://doi.org/10.3389/fmicb.2021.728173

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Abstract

Stem rot disease caused by Sclerotium rolfsii is one of the destructive diseases in peanut and poses a big risk to peanut production. Current fungicides in the market have not provided satisfactory control efficacy and thus called for novel fungicides with different structures as an alternative treatment strategy. Our previously developed phenylpyrazole compound 3c demonstrated modest inhibitory effect against S. rolfsii. The following structure modification identified an unreported compound 6, which bears a 3-chloropyridinyl moiety as the most prominent derivative with an IC50 of 12 μg/ml in potato dextrose agar (PDA) assay, higher than those of 0.8 and 1.8 μg/ml associated with thifluzamide and tebuconazole, respectively. However, compound 6 showed similar controlling effects to those of thifluzamide and tebuconazole in field study. This study underscores the potential of 1,5-diaryl-pyrazole-3-formate as an antifungal candidate for stem rot disease management.

Highlights

Stem rot disease is one of the destructive diseases in peanuts and leads to significant loss to peanut yields (Punja, 1985)
Melting points were determined with an X-4 melting points apparatus (Shanghai) and were uncorrected. 1H NMR and 13C NMR spectra were recorded on a Bruker AM-400 spectrometer at 400 and 100 MHz, respectively
Analytical high performance liquid chromatography (HPLC) was performed on the Thermo Ultimate 3000 equipped with Thermo Scientific Acclaim-C18 column and UV wavelength set at 254 nm

Summary

INTRODUCTION

Stem rot disease is one of the destructive diseases in peanuts and leads to significant loss to peanut yields (Punja, 1985). Peanut stem rot disease has been developing rapidly throughout the Henan province of central China for the growing planting area and continuous cropping, and is identified as a major challenge for peanut production (Tang, 2009). Cultural controlling strategies such as crop rotation are generally not well adopted by peanut growers for the economic concerns and fungicide treatment still represents an effective management strategy for disease control. Compound 6 was found rivaling the positive control in management of stem rot disease in field study

MATERIALS AND METHODS

RESULTS AND DISCUSSION

DATA AVAILABILITY STATEMENT