Abstract
Superresolution microscopy has revealed the importance of nano-scale arrangement of proteins for the various cell functions such as synaptic transmission and immune function. In particular, superresolution microscopy by single molecule localization method has high spatial resolution and is promising for research on nano-scale distribution of protein of interest in cells. However, live cell superresolution imaging by the introduction of single molecule localization method is delayed due to insufficient photon number of fluorescence and cytotoxicity of reducing agent used for the switching of fluorescence.
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