Abstract
Objective: The current work intended towards the developed and validated UV spectrophotometric method for the estimation of Tiotropium Bromide in API and pharmaceutical formulation.
 Methods: The UV spectrum of tiotropium bromide in methanol shows maximum absorbance at 230 nm. Calibration curve method was used for the estimation of tiotropium bromide in API and pharmaceutical formulation.
 Results: Maximum absorbance obtained in 230 nm. Calibration curve plotted in concentration range 20-120 µm/ml exhibit the linearity relationship with line equation y=0.0057x+0.3319. The Accuracy was found to be 80-100.5%. The precision %RSD=0.1110, and the limit of detection(LOD)=4.12and limit of quantification (LOQ= 12.50. The method was found to comply all the validation parameters as per the ICH guideline in indicating the sensitivity of the method analyte.
 Conclusion: This method is used as satisfactory for the routine analysis of tiotropium bromide in API and pharmaceutical dosage forms.
Highlights
ResultsCalibration curve plotted in concentration range 20-120 μm/ml exhibit the linearity relationship with line equation y=0.0057x+0.3319
The Tiotropium Bromide chemically is (1 2, 4β, 5∝, 7β,)-7[(Hydroxydi-2-thieyl-acetyl) oxy]-9, 9-dimethyl-3-oxa-9-azoniatricyclo [3.3.1.0] [1]
Tiotropium is used as a maintenance treatment of chronic obstructive pulmonary disease (COPD)
Summary
Calibration curve plotted in concentration range 20-120 μm/ml exhibit the linearity relationship with line equation y=0.0057x+0.3319. The Accuracy was found to be 80-100.5%. The precision %RSD=0.1110, and the limit of detection(LOD)=4.12and limit of quantification The method was found to comply all the validation parameters as per the ICH guideline in indicating the sensitivity of the method analyte
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Current Pharmaceutical Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
