Development and validation of UV spectrophotometric method for determination of chlorpropamide in bulk and formulation

Abstract

The current work focuses on the development and validation of a UV spectrophotometric technique for determining chlorpropamide in bulk and formulation. According to ICH criteria, UV spectrophotometric method was validated for a number of parameters, including linearity, precision, accuracy, sensitivity, limit of detection (LOD), and limit of quantification (LOQ). The maximum wavelength of chlorpropamide was discovered to be 580 nm, and a colorimetric method was created for the quantitative estimation of colour and to quantify the absorbance of a particular wavelength of light by a particular solution. The maximal wavelength of chlorpropamide in the complex mixture was discovered to be 710 nm. The UV spectrophotometric technique and colorimetry are simple, sensitive, accurate, repeatable, and exact. Chlorpropamide in bulk can be determined effectively using the suggested method.