DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF CEFTAZIDIME IN BULK AND PHARMACEUTICAL FORMULATION

Abstract

The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV - Visible spectrophotometric method for the estimation o f ceftazidime in bulk and pharmaceutical formulation. The solvent used throughout the experiment was the mixer of methanol and water. Absorption maximum (λ max ) of the drug was found to be 266 nm. The quantitative determination of the drug was carried o ut a t 266 nm and Beer’s law was obeyed in the range of 2 - 20 μg/mL. The method was shown linear in the mentioned concentration s having line equation y = 0.044x with correlation coefficient of 0.999. The recovery values for ceftazidime ranged from 9 9.83 % - 100. 56 %. The relative standard deviation of six replicates of assay was less than 2 %. The percent relative standard deviation ( RSD % ) of in terday precision range was 0.275 – 0.420 % and in traday precision range was 0.222 – 0.418 %. The limit of detection and li mit of quantification was 0.079 μg/mL and 0.241 μg/mL. The percent relative standard deviation of robustness and ru ggedness of the method was 0.146 - 0.231 %. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determin ation o f the bulk drug as well as dosage formulation.