Abstract
This study describes the development and validation of an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for determination of cediranib in human plasma. The method was sensitive, selective and rapid for the analysis of cediranib. A liquid-liquid extraction method was used and chromatographic separation was performed on aC 18,UPLC, BEH TM column (50 × 2.1 mm, i.d. 1.7m, Waters, USA) with a run time of 2.6 minutes. Crizotinib was used as internal standard. Multiple reaction monitoring ion transitions used for detection were m/z 451.43 −→ 112.02 and m/z 450.0 −→ 260.0 for cediranib and internal standard, respectively. The linearity of the assay was found to be between 1-300 ng/mL for cediranib in human plasma. The intra- and inter-assay precision relative standard deviations did not exceed 11.52 and mean extraction recovery was found to be 74.74 ± 2.29.
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