Development and Validation of Three Spectrophotometric Methods for Simultaneous Determination of Paracetamol and Pamabrom in Bulk and Pharmaceutical Formulation

Abstract

Three simple, sensitive and cost effective spectrophotometric methods are developed for simultaneous determination of Paracetamol (PCM) and Pamabrom (PAM). The methods are dual wavelengt method (DWM), ratio difference method (RDM) and mean centering of ratio spectra method (MCR). In DWM 252.4 and 295.8 nm were selected for determination of PCM, where PAM had equal absorbance values. Similarly, 211 and 225.6 nm were selected to determine PAM, where PCM had equal absorbance values. In RDM The zero order spectra of PCM were divided by the spectrum of 10 µg/mL of PAM, the peak amplitudes of the ratio spectra were measured at 222 and 244 nm; and the zero order spectra of PAM were divided by the spectrum of 20 µg/mL of PCM and the peak amplitudes were measured at 248 and 287 nm. In MCR the absorption spectra (210-260 nm) of PCM were divided by the spectrum of 10 µg/mL of PAM and the absorption spectra (210-260 nm) of PAM were divided by the spectrum of 20 µg/mL of PCM, the obtained ratio spectra were then mean centered and values at 245 and 215 nm were selected for determination of PCM and PAM respectively. The proposed methods were applied for analysis of PCM and PAM in pure form and pharmaceutical formulation. The developed methods were validated and met the requirements delineated by ICH guidelines with respect to linearity, accuracy, precision and specificity. The results prove the ability of proposed methods to be used for routine quality control analysis of these drugs.