Development and Validation of the UV-Spectrophotometric Method for Determination of Embelin

Abstract

Embelin, chemically known as 2, 5-dihydroxy-3-undecyl-p-benzoquinone is one of the bioactive compound found in oldest herbal medicinal plant known as Embelia ribes. Embelin, having anti-cancer, anti-bacterial, anti-inflammatory, analgesic, hepatoprotective, antidepressant, anxiolytic, antioxidant, antifertility, antiulcer and wound healing activity. A double wavelength UV-spectrophotometric method was developed and validated for determination of embelin in Embelia ribes fruits. The standard solution of embelin in methanol shown maximum absorption at 289 nm. The response of embelin was linear over the range of 10-90 µg/ml with the correlation coefficient (R2) value of 0.9991. The accuracy of the method was checked by recovery experiment performed at three different levels, i.e., 50%, 100%, and 150%. The % recovery was found in the range of 98.96– 100.9%. The precision of the method was studied as an intraday; interday and repeatability. The % relative standard deviation (RSD) value 2 indicate that the method was precise. The Limit of detection (LOD) and Limit of quantification (LOQ) of embelin were found 3.96 μg/mL and 12 μg/mL respectively. The method was validated using parameters provided as per International Conferences on Harmonization (ICH) guidelines.