Abstract
A rapid, simple and sensitive spectrophotometric method for the determination of amoxicillin (AM) is described. The method is based on the previous sulphanilamide (SA) and sulphathiazole (STZ) diazotization in the medium of 0.6-0.7 M hydrochloric acid and their subsequent interaction with amoxicillin at pH = 10.5 with formation of yellow-colored azo compouds. Effective molar absorptivities at the absorbance maxima at 445 nm (SA) and 448 nm (STZ) for azo compounds were (1.74 ± 0,06)∆104 L×mol-1×cm-1 and (1.97 ± 0,05)∆104 L×mol-1×cm-1, respectively. Stoichiometric ratios of the components of azo compounds were determined using continuous variations method. Based on the optimum reaction conditions, new methods were developed. These methods allow to determine the amoxicillin in concentration range 1.3-32.9 mg×mL-1 with sulphanilamide and 0.7-27.4 mg×mL-1 with sulphathiazole. The methods were successfully validated for amoxicillin determination in tablets "Amoxil".
Highlights
Significant development of medicine, chemistry, and biology result in the increased use of biologically active substances, in particular, antibiotics, which occupy an important place in contemporary medical and veterinary practices
The method is based on the previous sulphanilamide (SA) and sulphathiazole (STZ) diazotization in the medium of 0.6–0.7 M hydrochloric acid and their subsequent interaction with amoxicillin at pH = 10.5 with formation of yellow-colored azo compouds
Based on the optimum reaction conditions, new methods were developed. These methods allow to determine the amoxicillin in concentration range 1.3–32.9 mg × mL–1 with sulphanilamide and 0.7–27.4 mg × mL–1 with sulphathiazole
Summary
Significant development of medicine, chemistry, and biology result in the increased use of biologically active substances, in particular, antibiotics, which occupy an important place in contemporary medical and veterinary practices. The monitoring of their content to evaluate the quality of finished drugs products and detecting the counterfeits is a very important task. Since antibiotics often cause side-effects and allergic reactions, it is advisable to control their content in biological fluids, as well as in foods (milk, chicken tissues) because of their extensive use in livestock production. It is important to control the Structural formula (2S,5R,6R)-6-{[(2R)-2-amino-2-(4-hydroxy-phenyl)acetyl]amino}-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0] heptane-24-carboxylic acid.
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