Development and Validation of Stability-Indicating RP-HPLC Method for the Estimation of Cuminaldehyde

Abstract

Cumin aldehyde is an herbaceous plant (Cuminumcyminum L.) volatile oil that used as a regular spice in kitchen foods. It also has some pharmacological properties such as analgesic, hepato-protective, anti-inflammatory, antibacterial, antimicrobial, antifungal, antiviral, antioxidant and anticancer. The key objective of the present study was to develop and validate the RP-HPLC method for the estimation of cumin aldehyde. According to the ICH guidelines, RP-HPLC stability indicating method was used in which reverse phase enable Cosmosil C18 column (250 × 4.6 mm, 5μm) used in isocratic mode. For the estimation of cumin aldehyde, sodium sulphate: acetonitrile: methanol (20:73:7 v/v) was used as a mobile phase and it was delivered at flow rate of 1.0 mL min- 1. The injection volume was 20 µL and elute was analyzed by a UV detector at 326 nm. Linearity was observed between the concentration range of 20 μg mL-1 -140 μg mL-1 and the correlation coefficient R2 value was found to be 0.997 ± 0.5. The method was accurate and recovery was found to be in the range of 98.06 -100.40 %. The limit of detection of cumin aldehyde was found to be 1.04 μgmL-1and limit of quantitation was found to be 3.16 μgmL-1. Cumin aldehyde was subjected to stress conditions including acidic, alkaline, neutral, oxidation, and dry heat degradation. Cumin aldehyde was more sensitive to acidic, dry heat and oxidative degradation and it is stable at alkaline conditions. The method was validated according to ICH guidelines.