Abstract
In present research a stability indicating reversed-phase high-performance liquid chromatography (RP-HPLC) was developed and validated for quantification of azacitidine and its degradants. The chromatographic isolation of azacitidine carried out using Phenominex C18 (150×4.6 mm, 5 μ) column, movable phase of 0.1% HCOOH in water and acetonitrile (30:70 v/v), at a flowing rate of 1-mL/min analyzed at 210 nm. The %recovery findings ranged between of 99.13–99.41%. The limit of detection (LoD), and limit of quantitation (LoQ)values were assessed from the rectilinear plot and were found to be 3.78 and 15.12 μg/mL. The regression equation of the linearity curve was found to be y = 851.76x + 310.38 and r2 value of 0.9998. The samples were subjected to stress degradation and characterized by three acid degradation products by liquid chromatography-mass spectrometry (LC-MS/MS) - DPI, DPII, and DPIII. The stationary phase and movable system attained excellent resolution as chromatographic isolation and structure identification was accomplished using LC-MS/MS fragmentation
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