Development and validation of spectroscopic simultaneous equation method for simultaneous estimation of betamethasone and luliconazole in synthetic mixture

Abstract

A simple, specific, accurate and precise spectrophotometric method was developed and validated for simultaneous estimation of Betamethasone and Luliconazole in Synthetic Mixture. The wavelength of estimation for Betamethasone was 243.20 nm and for Luliconazole was 225.00 nm. Beer’s law is obeyed in the concentration range of 10-50μg/ml and 20-100μg/ml and correlation coefficient of 0.999 and 0.999 for Betamethasone and Luliconazole respectively. The % recovery for Betamethasone and Luliconazole were found to be 100.49 % & - 100.68 % respectively. Intraday precision of Betamethasone and Luliconazole were found to be 0.10 %– 0.28 % and 0.11 % – 0.24 % RSD and Interday precision were found to be 0.27 %– 0.37 % and 0.23 %– 0.35 % RSD respectively. The proposed method was also evaluated by the Assay of Synthetic mixture containing Betamethasone and Luliconazole. The % Assay was found to be 100.60 % for Luliconazole and 100.52 % for Betamethasone. Validation of proposed methods was carried out according to ICH Q2R1Guidelines. The proposed methods were found accurate and reproducible for routine analysis of both the drugs in synthetic mixture.