Abstract
A novel ultra fast liquid chromatography (UFLC) method has been developed and validated for quantification of Buprofezin in Suspension Concentrate (SC) formulation, using Shimadzu, packed with C18 (250 mm × 4.6 mm, 5 μm) column. Mixture of acetonitrile:water (80:20 v/v) was used as mobile phase. The flow rate was kept 1.5 ml/min and detection was carried out at 250 nm. The linearity of proposed method was investigated in the range of 200-1002 PPM (r2=0.999). The percentage recovery found to be 99.87%. The % RSD value for precision study was <0.68 as per modified Horwitz equation as requirements by CIPAC. The developed method was found to be specific, linear, precise and accurate. This method is also useful for quantification of Buprofezin in their single or combination formulated products, environmental samples (soil, water), and agricultural products for pesticide residue analysis.
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