Abstract
Objective: The present study was aimed to develop a simple, sensitive and precise high performance liquid chromatographic (HPLC) method for the simultaneous estimation of curcumin and piperine and to implement the developed method for the estimation of curcumin and piperine in the nanoparticulate formulation.Methods: Method development was performed using various solvent, buffer-solvent ratios, at different flow rates for adequate separation of both drugs. The developed method was validated in accordance with the international conference on harmonization (ICH) guidelines. The developed method was implemented to estimate the amount of curcumin and piperine in the nanoparticulate formulation.Results: Chromatographical conditions were optimized, and the best chromatographical conditions with adequate resolution for curcumin and piperine was achieved using enable C18G reverse phase column, using a mobile phase combination of acetonitrile and phosphate buffer (pH 3)in a ratio of 70:30 v/v at a flow rate of 1.0 ml/min. The detection was monitored at a wavelength of 360 nm. The retention time of curcumin and piperine was found to be 7.2 min and 8.5 min respectively.Conclusion: The developed analytical method is simple, precise, and reproducible and thus can be used for simultaneous estimation of curcumin and piperine in pharmaceutical formulations.
Highlights
IntroductionIt is a hydrophobic polyphenolic substance isolated from the rhizomes of Curcuma longa Linn
The method development for estimation of curcumin and piperine was carried out using various mobile phases including acetonitrile: 0.1% orthophosphoric acid, methanol: water and acetonitrile: water to achieve separation of curcumin and piperine
Accurate and cost-effective reversed-phase high performance liquid chromatographic (HPLC) method was developed for the simultaneous estimation of curcumin and piperine with mobile phase degassed mixture of acetonitrile: phosphate buffer pH 3 (70:30 v/v) at 1.0 ml/min flow rate which is suitable for column efficiency
Summary
It is a hydrophobic polyphenolic substance isolated from the rhizomes of Curcuma longa Linn. 1 along with other two de-methoxy compounds which are desmethoxycurcumin and bisdemethoxycurcumin [1, 2]. Safety of curcumin at very high doses has been proved in various animal and human studies. These studies led to the approval of curcumin as a ‘generally regarded as safe (GRAS)’ ingredient by the food and drug administration (FDA) of the United States of America, by the natural health products directorate of Canada and the expert joint committee [6]
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