Abstract
A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for estimation of Ethacridine lactate in pharmaceutical formulation. The method was carried out on a Qualisil RP C-18 (250mm × 4.6mm, 5pm) column with a mobile phase consisting of methanol: water (60:40v/v), pH adjusted to 2.8 with ortho- phosphoric acid and flow rate of 1.0mL/min. Detection was carried out at 271nm. The retention time for ethacridine lactate was found to be 4.41min. The ethacridine lactate followed linearity in the concentration range of 2–12μg/mL (r2=0.9980). The amount of the drug estimated by proposed method was found to be in good agreement with label claim. The developed method was validated for sensitivity, accuracy, precision, ruggedness and robustness. The LOD and LOQ were found to be 0.11 and 0.33μg. The proposed method can be used for routine analysis of ethacridine lactate in bulk drug and pharmaceutical formulation.
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