Abstract
Soursop leaves contain alkaloids that have pharmacological effects. This study aims determine the total alkaloid content of soursop leaf ethanol extract using a validated HPLC method. Extraction was carried out by soxhletation followed by liquid-liquid extraction using chloroform. Optimizing HPLC conditions on the mobile phase, flow rate, concentration, and wavelength, further testing the validation of the analytical method. Optimizing HPLC conditions obtained optimal results at concentration of 500 ppm with a flow rate of 1.0 mL/min using acetonitrile: methanol: water (80:5:15) at wavelength of 272 nm. These results have met the validation requirements of analytical methods including System Suitability Test (UKS), selectivity, linearity with y = 2.01914x –219,97226, correlation coefficient value (r) = 0.9995, and Vx0 value 0.11201%. The limit of detection and quantization were 8,78525 ppm and 29,28418 ppm, respectively. The RSD percentage of the precision test is 1.0453%, and the accuracy test is obtained from the recovery of 98-102%. The result of determining the total alkaloid content sample was 0.0862 ± 0.004 % (%w/w) of the entire alkaloid content (TALC) triple replication. This research concludes that the analytical method using the HPLC system for determining the total alkaloid content of the ethanolic extract of soursop leaves is validate
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
