Abstract
Objective: The main aim of the present study was to develop and validate a simple, precise and accurate Reversed-Phase HPLC-PDA method for the quantitative determination of Chrysin in solid lipid nanoparticles (SLNs).
 Methods: The RP-HPLC-PDA system equipped with a C-18 reversed-phase column (250 × 4.6 mm, particle size 5 μm) was employed in the present study. HPLC grade methanol and water in 85:15 (v/v) ratio was selected as the mobile phase at flow rate of 1 ml/min under an ambient column oven temperature. The detection wavelength was kept at 268 nm. Validation of developed method was performed according to the ICH guidelines.
 Results: The developed reversed-phase HPLC-PDA method was found to be linear in the concentration range of 0.2-10 µg/ml with a correlation coefficient of 0.999. The method was also observed to be precise with % relative standard deviation (RSD) below 2%. The limit of detection and limit of quantification of this method were found to be 0.05µg/ml and 0.14µg/ml, respectively. The percent recovery of the developed method was estimated to more than 99%.
 Conclusion: The developed HPLC method can be utilized for the determination of Chrysin with a high degree of accuracy, precision, robustness, specificity in solid lipid nanoparticles in the presence of excipients.
Highlights
Chrysin (5, 7-dihydroxyflavone) is a flavonoid and a primary active chemical constituent of the Indian trumpet trees (Oroxylum indicum). It is found in chamomile, in the mushroom Pleurotus ostreatus, propolis and in honey [1]
Previous studies revealed the presence of high Chrysin content in propolis while the Chrysin content in honeydew and forest honey was found to be 0.10 mg/kg and 0.53 mg/kg, respectively [2,3]
The therapeutic potential of Chrysin is severely limited by its poor oral bioavailability (F ≤ 0.02%) which is because of its poor aqueous solubility (2-5 μg/ml) and extensive pre-systemic metabolism in the intestine and the first-pass metabolism in the liver [6,7,8]
Summary
Chrysin (5, 7-dihydroxyflavone) is a flavonoid and a primary active chemical constituent of the Indian trumpet trees (Oroxylum indicum). Chrysin loaded solid lipid nanoparticles (Ch-SLNs) has been developed and evaluated for their physiochemical characterization and in vitro anticancer activity against MCF-7 breast cancer cells by the authors in their previous work. No reports were available as yet on reversed-phase HPLC equipped with photodiode array detector (RP-HPLCPDA) based methods for the quantification of Chrysin in solid lipid nanoparticles.
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