Abstract
A simple, precise, accurate, and rapid HPLC method was developed to estimate the amount of lamotrigine in bulk and its pharmaceutical formulations. Waters Alliance HPLC system equipped with autosampler, ultraviolet detector, and Symmetry C8 (4.6 mm ID × 150 mm, 3.5 μm, Make: XTerra) column were used for the quantification of the drug. Separation was carried out at a flow rate of 0.7 mL/min. of mobile phase (acetonitrile and potassium dihydrogen phosphate buffer of pH = 7.0 in the ratio 60 : 40 v/v), and the detection was carried out at a wavelength of 215 nm. The retention time of lamotrigine was found to be 2.797 min. The linearity was obeyed in the range of concentration 5–25 μg/mL. The developed method was found to be repeatable and reproducible; hence, it can be used as an alternative method in assay of the lamotrigine in any pharmaceutical industries.
Highlights
Lamotrigine (LTG) (Lamictal by Glaxo SmithKline) is an anticonvulsant drug used in the treatment of epilepsy and bipolar disorder
It is generally accepted to be a member of the sodium channel blocking class of antiepileptic drugs [1], but it could have additional actions in as much as it has a broader spectrum of action than other sodium channel antiepileptic drugs such as phenytoin and carbamazepine and is effective in the treatment of the depressed phase of bipolar disorder, whereas other sodium channel blocking antiepileptic drugs are not
Simultaneous determination of lamotrigine, zonisamide, and carbamazepine in human plasma by high-performance liquid chromatography was reported by Griner-Sosanko et al [7]
Summary
Lamotrigine (LTG) (Lamictal by Glaxo SmithKline) is an anticonvulsant drug used in the treatment of epilepsy and bipolar disorder. Emami et al [3] developed a HPLC method for determination of lamotrigine and related compounds in tablet formulations. Sallustio and Morris [6] reported a high-performance liquid chromatography method for quantitation of plasma lamotrigine concentrations in patients with epilepsy. Simultaneous determination of lamotrigine, zonisamide, and carbamazepine in human plasma by high-performance liquid chromatography was reported by Griner-Sosanko et al [7]. A spectroscopic method [20] in UV region (307 nm) was developed for the quantitative determination of lamotrigine in bulk and in dosage form in which linearity obeyed in the concentration range 5–50 mcg/mL. A few visible spectrophotometric methods [21,22,23] were developed for the determination of lamotrigine (LTG) in pharmaceutical dosage forms and urine samples using some chromogenic reagents. A few visible spectrophotometric methods [21,22,23] were developed for the determination of lamotrigine (LTG) in pharmaceutical dosage forms and urine samples using some chromogenic reagents. e aim of the present study was to develop and validate rapid, simple, and selective liquid chromatography method for LTG quality control in tablets
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