Abstract
The recent trend in green analytical chemistry is the development of green analytical methods using environmentally friendly solvents. Therefore, three ecofriendly manipulated UV spectroscopic techniques have been validated for the concurrent quantification of newly approved remogliflozin etabonate (REM) and metformin HCl (MET) tablets using water as a solvent. The first method was established using first derivative absorption spectroscopic method by determining the peak amplitude at 233.0 nm for REM and 252.2 nm for MET, a zero crossing of one the component. The second and third methods were based on the peak amplitude difference and first-order derivative absorption of the ratio spectra developed by the manipulation of scanned UV spectra. REM and MET showed good linearity in the series of 1–20 µg ml−1 and 2.5–35 µg ml−1, respectively, by all three methods with an excellent correlation coefficient (r2 ≥ 0.998). Further, the proposed UV spectroscopic techniques were validated as per International Council for Harmonization guidelines. The methods showed good sensitivity, accuracy, and precision. Anticipated procedures were effectively utilized for the concurrent quantification of REM and MET in laboratory prepared mixtures and tablets. The high percent recovery with low standard deviation found for both analytes by all three methods confirms the accuracy and precision of the procedures. Finally, the greenness of the proposed spectroscopic methods, evaluated by semi-quantitative and quantitative methods, showed the eco-friendly nature of the methods. Furthermore, the proposed approaches were simple, accurate, sensitive, economic, and environmentally friendly and hence can be utilized for regular quality control of REM and MET formulation.
Highlights
metformin HCl (MET) has UV absorption in the wavelength 200 to 260 nm with λmax 232.9 nm, and it is completely overlapped with the UV absorption of remogliflozin etabonate (REM), (Figure 2A) which has two λmax at 229.9 and 276.6 nm
Accurate, UV spectroscopic methods were established for concurrent quantification of REM and MET without the use of any complicated mathematical processing
The applicability of the current methods could be assessed by analyzing both analytes in laboratory prepared solutions in different ratios and in tablets with excellent accuracy and precision
Summary
Like Diabetes Mellitus Type 2 (DMT2), are already enormous and growing at alarming percentile throughout the world. The number of diabetic patients will increase to more than 500 million by the year 2030 and to more than 700 million by 2045 [1,2]. Numerous studies have been undertaken to elucidate more efficacious antidiabetic drugs with improved glycemic control, with fewer adverse effects [3]. Sodium-glucose cotransporter-2 inhibitors (SGLT-2) were recently approved for the management of DMT2, [4,5] alone or in combination with other antidiabetic drugs.
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