Abstract
Difference spectroscopic method is based on the principle that tolperisone hydrochloride can exhibit two different absorption spectra in basic and acidic medium. A stock solution (1 mg/ml) was prepared with distilled water. Further dilution was made by using 0.1 N sodium hydroxide and 0.1 N hydrochloric acid separately. The maxima and minima in the difference spectra of tolperisone hydrochloride were at 231nm and 263nm, respectively. Difference in absorbance between these maxima and minima was calculated to find out the amplitude. This amplitude was plotted against concentration. Linearity was found in the concentration range of 10-50µg/ml. The LOD and LOQ of tolperisone hydrochloride were found to be 0.064µg/mL and 0.196µg/mL respectively. The percentage recovery study of the drug for the proposed method was found in the range of 99.31 - 99.94% for tolperisone hydrochloride. The proposed method is recommended for routine analysis since it is rapid, simple, precise and accurate.
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