Abstract
Gangliosides are sialic acid containing glycosphingolipids of the plasma membrane with diverse biological functions. They are most abundant in neural tissues where their dysregulation has been suggested to be involved in various pathological conditions. Due to their importance, efficient analytical methods are needed to determine individual gangliosides in biological samples. Here we report a capillary electrophoresis method, optimized and validated for the simultaneous quantification of major neural gangliosides GM1, GD1a, GD1b, GT1b and GQ1b in their underivatized form. The most abundant extraneural monosialogangloside, GM3 can also be separated by this method. Micelles of the highly amphiphilic gangliosides were disrupted with cyclodextrins (CyDs) in the aqueous separation buffer. Among the tested CyDs, the best resolution was observed using 20 mM randomly methylated alpha-CyD in alkaline sodium borate buffer enabling the separation of all studied gangliosides. The method was applied for the quantification of gangliosides in rat cerebral and cerebellar synaptosomes.
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