Development and validation of BLRI Mastitis Test Kit at Bangladesh Livestock Research Institute Regional Station, Sirajganj.

Md Kabir,Razia Khatun,Md Yousuf,Md Giasuddin,Mohammad Islam,Nathu Sarkar,Md Sarker,Md Ershaduzzaman,K Nazir,Yousuf Ali

doi:10.5455/javar.2019.f363

Abstract

Objective:The objective of this study was to develop a low-cost kit for the detection of subclinical mastitis (SCM) and to check its validity, reproducibility, and efficacy at the field level.Materials and Methods:A total of 550 quarter milk samples from crossbred dairy cows were collected, of which 400 milk samples were used to validate the newly developed BLRI mastitis test (BMT) kit to justify its efficacy as an individual test kit in detecting SCM based on somatic cell count (SCC) by direct microscopic count (DMC). The efficacy of the newly developed BMT was compared with the California Mastitis Test (CMT) kit. Another 150 milk samples were subjected to SCC determined by DMC and DCC (De Laval cell counter®) categorized by CMT and BMT scores.Results:A SCM test kit, namely, BMT kit was successfully developed in this study. The percentage accuracy of CMT and BMT were 76.75% and 75.75%; sensitivity 69.36% and 67.56%; specificity 85.95% and 85.85%; positive predictive value 86.03% and 85.71%; negative predictive value 69.23% and 68%, respectively. A p value of 0.001 was found for both CMT and BMT. However, CMT and BMT had no significant difference in sensitivity (p = 0.778). Average SCCs (cells/ml) determined by DCC and DMC, respectively, were mostly corresponded to the SCC ranges of both CMT and BMT scores.Conclusion:The newly developed BMT kit is an independent, cheap, farmer-friendly, first country made, and reliable SCM diagnostic test kit that can be used at field condition.

Highlights

Mastitis is considered as one of the most common diseases causing economic losses due to reduced milk production, increased labor costs, increased treatment costs, animal death, and premature culling [1,2]
Materials and Methods: A total of 550 quarter milk samples from crossbred dairy cows were collected, of which 400 milk samples were used to validate the newly developed Bangladesh Livestock Research Institute (BLRI) mastitis test (BMT) kit to justify its efficacy as an individual test kit in detecting subclinical mastitis (SCM) based on somatic cell count (SCC) by direct microscopic count (DMC)
A total of 400 quarter milk samples from 100 crossbred dairy cows were subjected to BMT to justify its efficacy to validate as an individual test kit as compared to California Mastitis Test (CMT) in detecting SCM based on SCC through DMC

Summary

Introduction

Mastitis is considered as one of the most common diseases causing economic losses due to reduced milk production, increased labor costs, increased treatment costs, animal death, and premature culling [1,2]. Subclinical mastitis (SCM) shows no gross clinical signs in the udder of animals. This condition acts as a continuous source of infection for other herd mates. In Bangladesh, SCM causes great loss in the dairy industry, which estimates BDT 122.6 (US$ 2.11) million annually [6]. Several researchers have reported the prevalence, potential risk factors, and comparison of different screening tests for bovine mastitis in Bangladesh [10,11,12,13,14,15,16,17]. Development and validation of a cheap SCM screening kit is time demanding for saving foreign currencies and sustainable development of the dairy industry in Bangladesh.

Ethical statement

Results and Discussion

Conclusion

Conflict of interest