DEVELOPMENT AND VALIDATION OF BIOANALYTICAL RP HPLC METHOD FOR THE ESTIMATION OF METOPROLOL TARTRATE IN RABBIT PLASMA AFTER TRANSDERMAL AND ORAL ADMINISTRATION: APPLICATION IN PHARMACOKINETIC STUDIES

Abstract

A simple, specific, sensitive and rapid Reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the quantification of Metoprolol Tartrate in small volumes of rabbit plasma. The method was further extended for its pharmacokinetic studies in rabbit plasma samples after transdermal and oral administration. Biological sample preparation involving simple extraction with organic solvent, followed by dilution with mobile phase was adopted to eliminate any chromatographic solvent effects. The method was proven to be linear over a plasma concentration range of 20 ng/ml to100 ng/ml with a mean correlation coefficient of 0.99. The limit of detection and the limit of quantification of the newly developed method were determined to be 5.8ng/mL and 16.1ng/mL, respectively. The method was successfully applied to assess pharmacokinetic parameters of Metoprolol Tartrate in rabbit plasma and found out the comparative bioavailability of MT following oral and transdermal dosage forms. The developed method was established as a rapid analytical tool in a pharmacokinetic study as it required short retention time, high precision, sensitivity and small volumes of plasma for analysis. Keywords : Metoprolol Tartrate, RP-HPLC, quantification, Rabbit plasma, Pharmacokinetic study, oral, transdermal.