Development and Validation of Analytical Standards of Coconut (Cocos nucifera Linn.) shell based on Pharmacognostic, Phytochemical and Chromatographic Techniques

Abstract

Background: Cocos nucifera Linn. belongs to the Arecaceae family and is the only accepted species from the genus Cocos. Coconut shell is a usually discarded material from the plant source Cocos nucifera Linn. However, scientifically validated data regarding the chemical and conforming therapeutic profile of Coconut shell is not available till date. It is important to develop analytical standards of Coconut shell using pharmacognostic, phytochemical and chromatographic techniques. Materials and Methods: The present analytical study was designed to determine the organic compounds present in the active fraction of dried ripe Coconut shell and to develop drug standards using powder microscopy, quantitative and qualitative phytochemical analysis and TLC techniques. Results: Powder microscopy and preliminary phytochemical analysis of Cocos nucifera Linn. endocarp indicated the presence of high fibre content in the drug. Further studies revealed the test drug as a rich source of alkaloids, steroids, phenols, flavonoids and tannins. Bioactive chemical profile and analytical standards of the test drug were derived by Thin Layer Chromatographic studies as well. Conclusion: The findings of the current research work ensued in establishing the botanical and analytical standards for Coconut (Cocos nucifera Linn.) shell.