Abstract
Nateglinide has been widely used in the treatment of type-2 diabetics as an insulin secretogoga. A reliable, rapid, simple and sensitive reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for determination of nateglinide in rabbit plasma. The method was developed on Hypersil BDSC-18 column (250mm×4.6mm, 5mm) using a mobile phase of 10mM phosphate buffer (pH 2.5) and acetonitrile (35:65, v/v). The elute was monitored with the UV–vis detector at 210nm with a flow rate of 1mL/min. Calibration curve was linear over the concentration range of 25–2000ng/mL. The retention times of nateglinide and internal standard (gliclazide) were 9.608min and 11.821min respectively. The developed RP-HPLC method can be successfully applied to the quantitative pharmacokinetic parameters determination of nateglinide in rabbit model.
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