Abstract
New, simple, cost effective, accurate and reproducible RP-HPLC method was developed and validated for the quantification of trans-resveratrol in the extracts of grape exocarp and seeds. The method has proved to be simpler and faster than available methods. Methanol was used as a mobile phase with a flow rate of 1.0 cm3 min-1, while the quantification was effected at 306 nm. The separation was performed at 35?C using a C18 column. The results showed that the peak area response was linear in the concentration range of 1-40 ?g cm-3. The values of LOD and LOQ were found to be 0.125 and 0.413 ?g cm-3, respectively. The antioxidant activity of the extracts was determined using DPPH assay. The ability of DPPH radicals inhibition decreases in the following order: the extract of grape exocarp > trans-resveratrol standard > the extract of grape seeds.
Highlights
New, simple, cost effective, accurate and reproducible RP-HPLC method was developed and validated for the quantification of trans-resveratrol in the extracts of grape exocarp and seeds
The chromatographic conditions were optimized to provide a good performance of the assay
These results revealed that any small change in trans-resveratrol concentration in the solution could be accurately determined by the proposed analytical method
Summary
Simple, cost effective, accurate and reproducible RP-HPLC method was developed and validated for the quantification of trans-resveratrol in the extracts of grape exocarp and seeds. Based on the literature search, the HPLC method for simultaneous determination of the resveratrol content and other compounds (e.g., phenolic compounds in the plant extracts or metabolites in the biological samples) are mainly developed The combined solvents such as methanol, acetic acid, water, acetonitrile, etc. Given the state of the literature, the aim of this study was to develop simple, precise, accurate and validated RP-HPLC method for the determination of the trans-resveratrol content in the extracts of grape exocarp and seeds in the presence of other bioactive compounds. These plant materials were used because they represent the main source of this bioactive compound.
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