Abstract
This research describes the development and validation of a sandwich-type Enzyme-Linked-Immunosorbent Assay (ELISA) assay based on a sheep polyclonal antibody developed to determine porcine immunoglobulin G (IgG) concentration in serum samples. The coating and blocking conditions were established. The immunoassay demonstrated the ability to specifically quantify pig IgG in serum samples without reacts with rabbit or mouse IgG, evaluated in serum and ascites, respectively. Given its precision, specificity and accuracy, this ELISA is a tool for the measurement of IgG in porcine serum samples, to evaluate the humoral immune status of pigs.
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