Abstract
Analysis of fixed dose combination products can present daunting challenges to the analytical chemist. This paper presents a validated analytical method for simultaneous analysis of lamivudine, tenofovir disoproxil fumarate, and efavirenz using high-performance thin-layer liquid chromatography (HPTLC)-densitometric method. Separation was achieved by use of HPTLC pre coated plate with silica gel 60F254 using mobile phase containing toluene-methanol (27:6 v/v). RF values were 0.18 ± 0.02 for lamivudine, 0.33 ± 0.05 for tenofovir disoproxil fumarate, and 0.48 ± 0.02 for efavirenz, respectively. The regression line had best fit using second order polynomial function for all the three APIs (active pharmaceutical ingredients) with r2 ≥ 0.98 at the concentrations of 375–900 ng spot−1 for lamivudine, tenofovir disoproxil fumarate and 750–1800 ng spot−1 for efavirenz, respectively. Repeatability and intermediate precision had % RSD ≤ 2. The method had acceptable level of accuracy for all three APIs with mean recov...
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
