Abstract
An accurate and simple HPLC-UV method has been developed for the determination of clonidine in mouse plasma. A reversed phase C18 Nova Pack® column (125 mm × 4.6 mm i.d., × 3 μm particle size) was used as stationary phase. The mobile phase composition was a mixture of 0.1% diethylamine/acetonitrile (70:30, v/v) at pH 8 in an isocratic mode at flow rate was 1.0 mL/min. Detection was set at 210 nm. Tizanidine was used as an internal standard. The clonidine and tizanidine were extracted from plasma matrix using the deproteinization technique. The developed method exhibited a linear calibration range 100.0–2000 ng/mL and the lower limit of detection (LOD) and quantification (LOQ) were 31.0 and 91.9 ng/mL, respectively. The intra-day and inter-day accuracy and precision of the method were within 8.0% and 3.0%, respectively, relative to the nominal concentration. The developed method was validated with respect to linearity, accuracy, precision, and selectivity according to the US Food and drug guideline. Minimal degradation was demonstrated during the determination of clonidine under different stability conditions. The suggested method has been successfully applied during a pharmacokinetic study of clonidine in mouse plasma.
Highlights
Clonidine is the imidazoline derivative with the chemical name of2-[2,6-dichlorophenyl)imino]imidazoline HCl [1], which acts as a hypotensive agent (Figure 1).Clonidine belongs to a class of medications: alpha-adrenergic central agonists [2]
HPLC-UV detection is widely used in quality control, pharmaceutical analysis, and clinical
HPLC-UV detection is widely used in quality control, pharmaceutical analysis, and clinical advanced techniques (e.g. HPLC-MS or gas chromatography-mass spectrometry (GC-MS))
Summary
2-[2,6-dichlorophenyl)imino]imidazoline HCl [1], which acts as a hypotensive agent (Figure 1). Clonidine belongs to a class of medications: alpha-adrenergic central agonists [2] It acts as a hypotensive agent and is used to treat blood pressure disorders, hyperactivity disorders, addiction migraine headaches, and to manage opium and alcohol withdrawal symptoms [3]. A simple andcost lowdetection cost detection method a reasonable selectively sensitivity, will offer wider applicability in various analytical laboratories. To the best of our knowledge, the use of HPLC-UV detection of clonidine in mouse plasma chromatography. To the best of our knowledge, the use of HPLC-UV detection of clonidine in run mouse plasma in a method was 8 minutes the been collected were robust, exhibiting a high degree of precision. Method was 8ofminutes and the were robust, a high degree of precision Applicability of this method in a pharmacokinetic study was demonstrated
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